Movies
The images on this page are projected stacks of single slice images which have been animated to show the structure of the cardiomyocyte in three dimensions. While they are not as clear as the original images from the microscope, they do give an impression of the myofibrillar structure inside of a cardiomyocyte.
Since the size of some of these movie files can get quite large, so downloading them is not for the bandwidth-impaired crowd. I have indicated the size of the files blinking on and off at the end of each description so you can decide if you have the connection speed to handle the movie. Alternatively, you could start downloading the file, go have a nice meal with dessert, and the movie should be ready to play when you get back to the computer. Just click on the title for the DIC videos or the pictures of the cardiomyocytes to start the download.
Follow the instructions for
installing the player. You should then be able to select one of the
following movies to view:
Adenovirus DIC video- Real-time differential interference
contrast microscopy of neonatal rat cardiomyocytes showing the effect
of altering tropomodulin expression upon myofibril organization and
beating. .
Doxorubicin DIC video- Real-time differential interference
contrast microscopy of neonatal rat cardiomyocytes showing the effect
of doxorubicin upon upon myofibril organization and beating. .
A normal cardiomyocyte labeled
with antibodies to alpha-actinin (red) and tropomodulin (green) rotated +
30 degrees off axis from the X-Y plane of original viewing. The close
packing and parallel alignment of myofibrils is clearly evident in this
cell. .
Another normal cardiomyocyte as described
above. .
A cardiomyocyte from a transgenic mouse
showing accumulation of tropomodulin. This cell shows accumulation of
tropomodulin reactivity which appears to accululate in patches along
the length of the myofibrils. This cell is likely to have been in the
process of myofibril degeneration. .
This page and its contents are in a slow process of being tested and improved. If you have specific questions of comments, please send us e-mail through the link at the home page.
All images of cardiomyocytes on this
and linked pages are the property of Mark Sussman, Ph.D. and cannot be
legally copied, transferred, or reproduced in any way without owner
consent.